汉恒生物科研团队现已全面推出腺相关病毒(AAV)包装技术服务,并有多达12种的血清型载体及多种汉恒专业改造的突变载体可供选择,特异性地针对不同的组织器官。专业的科研团队,一流的技术服务,欢迎来电咨询,全国免费技术服务热线:400-092-0065
AAV其他相关特色服务
1. AAV在心肌中的应用
2. AAV在肝脏组织中的应用
3. 穿越血脑屏障AAV
5. AAV光遗传技术服务
6. AAV在眼科学中的应用
8. AAV在肺组织中的应用
9. AAV在脂肪组织中的应用
10. AAV在脑组织的应用
AAV特异性感染器官及组织的效果图
——更多组织器官的感染详情请咨询汉恒技术工程师 免费热线:400-092-0065
汉恒AAV感染小鼠脑干 汉恒AAV感染小鼠海马 汉恒AAV感染小鼠心肌 汉恒AAV感染小鼠肝脏
汉恒AAV感染大鼠肺脏 汉恒AAV感染小鼠视网膜 汉恒AAV感染小鼠骨骼肌
产品简介
腺相关病毒(Adeno-Associated Viral Vector,AAV)属于微小病毒科( parvovirus),为无包膜的单链线状 DNA 病毒。AAV 的基因组约 4700bp,包括上下游两个开放读码框架(ORF),位于分别由 145 个核苷酸组成的 2 个反向末端重复序列(ITR)之间。 由于腺相关病毒具有宿主范围广、安全性高、免疫原性低、表达稳定和物理性质稳定等优点,已被广泛地应用于基础研究和临床试验中,并且腺相关病毒载体已成为世界上最常用的基因治疗载体之一。
图 1. AAV 基因组结构
AAV病毒特点
1. 安全性高:迄今从未发现野生型 AAV 对人体致病,重组 AAV 基因组序列上去除了大部分的野生型 AAV 基因组元件,进一步保证了安全性;
2. 免疫原性低:AAV2 的基因组仅 4681 个核苷酸,便于用常规的重组 DNA技术进行操作,而且进行动物实验时造成的免疫反应小;
3. 宿主范围广:能感染分裂细胞和非分裂细胞;
4. 表达稳定:能介导基因的长期稳定表达;
5. 物理性质稳定:在 60℃不能被灭活,能抗氯仿;
AAV生物学特性
表1 不同病毒的生物学特性比较
AAV载体不同血清型
研究发现 AAV 具有多种血清型,各种不同血清型的 AAV 载体的主要区别是衣壳蛋白不同,因此对不同的组织和细胞的转染效率存在差异。目前汉恒生物在包装腺相关病毒时有 12种不同的 AAV 血清型可供客户选择,建议客户针对不同组织器官选择相应血清型的 AAV 病毒,见表 2。
表2. 12种不同血清型AAV对各组织器官细胞的亲和性
服务流程
AAV应用介绍
A. AAV9感染小鼠脑组织(PMID: 23503602)
B. AAV2感染大鼠脑组织
C. AAV2感染肾脏(PMID: 23216315)
D. AAV感染心脏(PMID: 20703310)
E. AAV9感染骨骼肌(PMID: 21811247)
F. AAV感染肝脏(PMID: 19861950)
参考文献
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